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Vitamin D Test Cost: Compare 5 Prices
How much does the Vitamin D (25-hydroxy) cost? We found 5 posted prices from 5 sellers, from $47.99 to $99 including separately listed fees. The cheapest vitamin D test price open to the public was $47.99 at TestWell. Last checked Oct 20, 2026.
What it measures
25-hydroxyvitamin D [25(OH)D] in blood.
Lab: Quest or Labcorp (varies by seller) · Specimen: Blood draw at a patient service center
Evidence level: Widely used, with known limits
The US Preventive Services Task Force (2021) concluded that evidence is insufficient to assess screening for vitamin D deficiency in adults without symptoms (I statement), noting that no single blood level defines deficiency and that assays can over- or underestimate 25(OH)D. The 2024 Endocrine Society guideline suggests against routine 25(OH)D testing in the general populations it considered, as distinct from people with established indications for testing.
Sources:
- US Preventive Services Task Force. Vitamin D Deficiency in Adults: Screening (2021). https://www.uspreventiveservicestaskforce.org/uspstf/recommendation/vitamin-d-deficiency-screening
- Demay MB, et al. Vitamin D for the Prevention of Disease: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2024;109(8):1907-1947. https://pubmed.ncbi.nlm.nih.gov/38828931/
Tiers: Established · Widely used, with known limits · Emerging · Not validated · Not yet reviewed. We publish a tier only when we can cite a guideline, systematic review or major professional-society statement. This summary describes published literature and is not a recommendation to take or skip any test.
Vitamin D Test prices, lowest total first
| Seller | Price | Listed fees | Total | Who can order | Notes | Links and actions |
|---|---|---|---|---|---|---|
| TestWellLOWESTSAVED Vitamin D (25-OH) | $41.99 | $6 lab processing fee (per order) | $47.99 | Self-order | View | |
| Direct Laboratory Services (DirectLabs)SAVED Vitamin D, 25-Hydroxy | $59 | $0 listed | $59 | Self-order | Quest Diagnostics draw. Price = DirectLabs catalog 'Reg Price' (DL_Price field of the store's public catalog API); the testinfo page does not display price. | View |
| Walk-In LabSAVED Vitamin D, 25-Hydroxy Blood Test | $59 | $6 physician fee (per order) | $65 | Self-order | Lab (Quest vs Labcorp) for this listing price not confirmed. | View |
| True Health LabsSAVED Vitamin D | $99 | $0 listed | $99 | Self-order | Quest. Product page says price includes the patient service center draw; Quest self-order not available in NY, NJ, MA, RI. (price read manually; site blocks automated checks) | View |
| Labcorp OnDemandSAVED Vitamin D Test | $99 | $0 listed | $99 | Self-order | Labcorp draw. | View |
Totals add only fees each seller lists on its product page (e.g., physician-authorization fees). Shipping, blood-draw, sales tax and state availability may vary. Links may become affiliate links in future; see our disclosure.
Vitamin D Test FAQ
How much does a vitamin D test cost?
On Oct 20, 2026, 5 posted prices from 5 sellers ranged from $47.99 to $99 including listed fees (median $65). The lowest price open to the public was $47.99 at TestWell.
What's included in the vitamin D test price?
Each row is the seller's listing for the Vitamin D (25-hydroxy) (lab: Quest or Labcorp (varies by seller); specimen: Blood draw at a patient service center). The Total column adds fees the seller lists on the product page: TestWell ($6 lab processing fee (per order)), Walk-In Lab ($6 physician fee (per order)). Shipping, tax, blood draws and consultations are not included unless the row says so.
Do you need a doctor's order for a vitamin D test?
Usually not. 5 of 5 listings here are marked self-order: you can buy without bringing your own doctor's order. Some add a listed physician or handling fee, included in the Total. Check the 'Who can order' column.
Which states restrict ordering a vitamin D test?
Sellers in our table note these limits: True Health Labs: Quest self-order not available in NY, NJ, MA, RI. State rules for consumer-ordered lab tests vary and sellers may block some states at checkout, so confirm on the seller's page.
Can I use an HSA or FSA to pay?
The IRS counts "laboratory fees that are part of medical care" as medical expenses (IRS Publication 502), and defines medical care as the diagnosis, cure, mitigation, treatment or prevention of disease. Whether a particular at-home test qualifies depends on that and on your plan's rules, so check with your HSA or FSA administrator before paying.
Why do prices differ so much?
Sellers set their own prices for the same lab's kit and may bundle practitioner authorization, consultations, shipping or add-ons. The table shows the price and any separately listed fees as posted on each seller's public page on the date checked.
How current are these prices?
All prices were checked on Oct 20, 2026. Prices and availability (including state restrictions) change; confirm on the seller's page before ordering.
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